Elements and Performance Criteria
- Receive samples and process associated request forms
- Check samples and request form details before they are accepted
- Return samples and request forms that do not comply with requirements to source with reasons for non-acceptance
- Log samples, recording details that allow accurate tracking and chain of custody
- Distribute samples for local testing or dispatch samples to other testing facilities
- Store samples appropriately where testing or transport is to be delayed
- Prepare for safe microbiological work and aseptic applications
- Select work area and equipment required for the safe handling of materials that may contain micro-organisms of specified risk groups
- Wear protective apparel, replacing it when contamination is suspected
- Apply correct disinfection procedures to work areas before and after use
- Locate relevant emergency equipment for timely response to microbiological accidents
- Apply standard precautions when handling biological materials
- Minimise the production and release of aerosols, using biological safety cabinets where necessary
- Clean spills, and report all spills and suspected incidents to supervisor
- Wash hands before and after laboratory work and when contamination is suspected
- Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with enterprise procedures
- Process samples for direct examination
- Prepare pure cultures for microbiological work and aseptic applications
- Select culture media to maximise growth of micro-organisms and cells
- Inoculate media aseptically, applying techniques suitable for purpose of culture
- Incubate inoculated media in conditions to optimise growth of organisms and cells
- Subculture on suitable media to optimise production of pure cultures
- Perform procedures that can assist in the identification of micro-organisms
- Select staining techniques to demonstrate required cellular characteristics
- Stain prepared films to demonstrate diagnostically useful characteristics
- Inoculate and incubate media with pure cultures to assist in the biochemical and immunological identification of micro-organisms
- Perform tests on pure cultures to assist in the biochemical and immunological identification of micro-organisms
- Estimate the number and/or size of micro-organisms in samples
- Count cells in undiluted samples to indicate the dilution necessary to reliably count organisms in culture
- Prepare serial dilutions of samples aseptically for culture and colony counting
- Count colonies for calculating number of viable organisms per unit volume
- Count micro-organisms in samples and cultures using spectrometric and electronic methodologies, where relevant
- Estimate and document uncertainty of measurement in accordance with enterprise procedures, where relevant
- Contribute to antibiotic sensitivity testing where required
- Prepare inoculum suitable for antibiotic sensitivity testing
- Dispense or position antibiotic discs as indicated by enterprise protocol
- Incubate inoculated media under conditions to maximise growth of cultured organism
- Read and record sensitivity reactions, noting phenomena that can assist in the correct interpretation of results
- Maintain records of laboratory work