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Elements and Performance Criteria

  1. Prepare for production
  2. Initiate and maintain cultures
  3. Harvest culture
  4. Complete post-culture production activities

Required Skills

Required skills

adding inoculation cultures and nutriments to production vessels according to enterprise procedures

communicating with supervisor on production activities and on own performance

communicating ideas on possible improvements

harvesting algal or livefeed cultures

maintaining axenic transfer of parent cultures

operating and maintaining production and harvest equipment

providing oral reports to supervisor on algal or livefeed production

recognising and reporting on obvious problems associated with the culturegrowing environment and culture health

removing waste and maintaining water levels according to enterprise procedures

using a microscope to examine culture for signs of health

Literacy skills used for

interpreting manufacturer guidelines

interpreting production schedules and labels

recording workplace data related to the production of algal or livefeed cultures

interpreting warning and safety signs

Numeracy skills used for

calculating culture periods

counting or determining density of algal or livefeed culture organisms

diluting to reach desired salinity

estimating weight of small quantities of chemicals

measuring chemical volumes and weights for sanitation

reading gauges and meters

Required knowledge

culture periods and life cycles of culture organisms

ESD principles

operation and maintenance of automatic or mechanised equipment

physiochemical requirements of the culture organisms

procedures for producing and harvesting algal or livefeed cultures

purpose of and operating procedures for algal or livefeed culture production and harvest equipment

purpose of nutriments

Evidence Required

The evidence guide provides advice on assessment and must be read in conjunction with the performance criteria required skills and knowledge range statement and the Assessment Guidelines for the Training Package

Overview of assessment

Critical aspects for assessment evidence required to demonstrate competence in this unit

Assessment must confirm the ability to

follow work procedures and supervisors instructions for the production process

produce algal or livefeed cultures under routine conditions

Assessment must confirm knowledge of

basic process for effective production of algal or livefeed cultures including setting up initiating and breeding monitoring health and growth harvesting and cleaning up activities

Context of and specific resources for assessment

Assessment is to be conducted at the workplace or in a simulated work environment It should cover the entire process of production from establishing the culture through to harvesting

Resources may include

inoculation cultures and nutriments

production schedule

production vessels

production and harvest equipment

records for documenting algal or livefeed culture production

treated water

work procedures for producing algal or livefeed cultures

Method of assessment

The following assessment methods are suggested

practical demonstration

project work

written or oral shortanswer testing

Guidance information for assessment

This unit may be assessed holistically with other units within a qualification


Range Statement

The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included.

Relevant government regulations, licensing and other compliance requirements may include:

biodiversity and genetically modified organisms

biosecurity, translocation and quarantine

business or workplace operations, policies and practices

environmental hazard identification, risk assessment and control

OHS hazard identification, risk assessment and control.

OHS guidelines may include:

appropriate workplace provision of first aid kits and fire extinguishers

clean, uncluttered, hygienic workplace

codes of practice, regulations and/or guidance notes which may apply in a jurisdiction or industry sector

enterprise-specific OHS procedures, policies or standards

hazard and risk assessment of workplace, maintenance activities and control measures

induction or training of staff, contractors and visitors in relevant OHS procedures and/or requirements to allow them to carry out their duties in a safe manner

OHS training register

safe lifting, carrying and handling techniques, including manual handling, and the handling and storage of hazardous substances

safe systems and procedures for outdoor work, including protection from solar radiation, fall protection, confined space entry and the protection of people in the workplace

systems and procedures for the safe maintenance of property, machinery and equipment, including hydraulics and exposed moving parts

the appropriate use, maintenance and storage of PPE.

ESD principles may include:

control of effluents, chemical residues, contaminants, wastes and pollution

improving energy efficiency

increasing use of renewable, recyclable and recoverable resources

minimising noise, dust, light or odour emissions

preventing live cultured or held organisms from escaping into environment

reducing energy use

reducing emissions of greenhouse gases

reducing use of non-renewable resources

undertaking environmental hazard identification, risk assessment and control

undertaking facility quarantine, biosecurity and translocation of livestock and genetic material

using and recycling of water, and maintaining water quality.

PPE may include:

hard hat or protective head covering

non-slip and waterproof boots (gumboots) or other safety footwear

protective eyewear, glasses and face mask

uniforms, overalls or protective clothing (e.g. mesh and waterproof aprons).

Algae or live-feed culture may include:

aquatic plants

brine shrimp (Artemia and Parartemia)

copepods

Daphnia

feeder fish, usually goldfish or other inexpensive fish (check state/territory animal welfare laws)

infusions and bio-fouling

inoculation or starter culture, usually an axenic monoculture

micro-algae and seaweed (various species)

mosquitoes, beetles or other insect (adult or larvae)

nematodes

polychaetes, oligochaetes and other worms

rotifers

zooplankton.

Production schedule may include:

culture method to be used:

batch

semi-continuous

continuous

enrichment of natural waters

production vessel or structure to be used

quality, including bacteria free, growth rate, size and age

quantities (i.e. cells/ml, organisms/ml) or weights

types of cultures, including species, sizes and ages

written instructions and work procedures.

Production vessel or structure may include:

concrete or earthen ponds

fibreglass or plastic tanks

glassware

plastic bags

plastic-lined pools.

Other equipment may include:

aeration

air filters

autoclave

bottle plugs

counters and microscopes

flexible tubing

greenhouses or hothouses

lights

measuring cylinders

pipettes and syringes

production vessel holder

temperature-controlled room

washing and sterilising equipment

water filtration equipment.

Treated water may include:

aeration

chemical (e.g. change pH and hardness)

chlorination and de-chlorination

heating or cooling

micro-filtration

pre-conditioning (e.g. left to stand with or without aeration)

ultraviolet (UV) and ozone.

Nutriments may include:

feeds, including micro-algae, pellets, powders and emulsions

enrichment formulae

green water

nutrients, fertilisers or other chemicals.

Physio-chemical requirements of the culture organism may include:

carbon dioxide

chemical, mechanical or biological filtration

dissolved oxygen

hardness

light

nitrogenous wastes and contaminants

pH

salinity

temperature

water flow.

Culture period may include:

one to several days for brine shrimp

several weeks for micro-algae.

Indicators of culture health may include:

colour

contaminants, including ciliates, males in rotifer cultures and unwanted species in micro-algal cultures

density of organisms (i.e. numbers per litre or millilitre)

feeding activity

flocculation of culture medium

growth

physical appearance

swimming activity.

Wastes may include:

dead or dying organisms

other contaminants, including soil and organic debris

uneaten nutriments.

Harvesting equipment may include:

collection vessels

continuous centrifuge

counters

live feeds:

buckets

nets, sieves or screens (mesh size generally below 100/mm)

micro-algal:

buckets

pumps

siphons

pipettes and measuring cylinders

washing sieves.