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Evidence Guide: ACMATE505A - Carry out advanced breeding procedures

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

ACMATE505A - Carry out advanced breeding procedures

What evidence can you provide to prove your understanding of each of the following citeria?

Establish breeding programs for production lines

  1. Institutional policies, procedures and protocols as set down by the AEC and relevant legislative requirements including, occupational health and safety (OHS), are complied with at all times.
  2. Genetic diversity principles are identified and related to breeding program requirements.
  3. Differences between knock in, knock out, transgenic and genetic drift lines are defined.
  4. Breeding programs for multiple generation production lines are designed and established.
  5. Data collection record systems are confirmed and/or designed and developed if required.
  6. Institutional standard operating procedures for breeding programs are reviewed and modified if required.
Institutional policies, procedures and protocols as set down by the AEC and relevant legislative requirements including, occupational health and safety (OHS), are complied with at all times.

Completed
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Teacher:
Evidence:

 

 

 

 

 

 

 

Genetic diversity principles are identified and related to breeding program requirements.

Completed
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Differences between knock in, knock out, transgenic and genetic drift lines are defined.

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Breeding programs for multiple generation production lines are designed and established.

Completed
Date:

Teacher:
Evidence:

 

 

 

 

 

 

 

Data collection record systems are confirmed and/or designed and developed if required.

Completed
Date:

Teacher:
Evidence:

 

 

 

 

 

 

 

Institutional standard operating procedures for breeding programs are reviewed and modified if required.

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Select animals and prepare for breeding

  1. Breeding program for specific production line requirements and production schedules are identified and confirmed.
  2. Animals for mating are selected and monitored to determine the current stage of their reproductive cycle.
  3. Females are monitored and identification for stages of oestrus cycle and those that are ready for mating are identified.
  4. Animals selected for mating are placed in an appropriate environment.
Breeding program for specific production line requirements and production schedules are identified and confirmed.

Completed
Date:

Teacher:
Evidence:

 

 

 

 

 

 

 

Animals for mating are selected and monitored to determine the current stage of their reproductive cycle.

Completed
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Females are monitored and identification for stages of oestrus cycle and those that are ready for mating are identified.

Completed
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Animals selected for mating are placed in an appropriate environment.

Completed
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Implement breeding procedures

  1. Appropriate mating systems and methods are selected and applied in accordance with the breeding program.
  2. Animals are monitored during and after the mating period to determine whether mating has been successful.
Appropriate mating systems and methods are selected and applied in accordance with the breeding program.

Completed
Date:

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Animals are monitored during and after the mating period to determine whether mating has been successful.

Completed
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Perform post-mating procedures

  1. Animals are monitored for signs of hatching or parturition.
  2. Fostering arrangements or caesarean rederivations are planned as necessary.
  3. Quality of offspring is assessed in accordance with required quality parameters of the breeding program.
  4. Offspring are indentified, sexed and weaned in accordance with the breeding program.
  5. Post-weaning management is carried out in accordance with the breeding program.
  6. Breeding records are maintained according to the breeding program requirements and institutional policies and procedures.
  7. Breeding program outcomes are reviewed and modified for future production runs as required.
Animals are monitored for signs of hatching or parturition.

Completed
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Fostering arrangements or caesarean rederivations are planned as necessary.

Completed
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Quality of offspring is assessed in accordance with required quality parameters of the breeding program.

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Offspring are indentified, sexed and weaned in accordance with the breeding program.

Completed
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Evidence:

 

 

 

 

 

 

 

Post-weaning management is carried out in accordance with the breeding program.

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Breeding records are maintained according to the breeding program requirements and institutional policies and procedures.

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Breeding program outcomes are reviewed and modified for future production runs as required.

Completed
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Evidence:

 

 

 

 

 

 

 

Name and record animals

  1. Types of animals are identified.
  2. The correct genetic names of animals are established by gathering information and seeking guidance from the relevant nomenclature committee and scientists.
  3. Strain names are registered if appropriate.
Types of animals are identified.

Completed
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The correct genetic names of animals are established by gathering information and seeking guidance from the relevant nomenclature committee and scientists.

Completed
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Strain names are registered if appropriate.

Completed
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Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Evidence Guide

The Evidence Guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package.

Overview of assessment

Critical aspects for assessment and evidence required to demonstrate competency in this unit

The evidence required to demonstrate competence in this unit must be relevant to workplace operations and satisfy all of the requirements of the performance criteria, required skills and knowledge and the range statement of this unit. Assessors should ensure that candidates can:

establish breeding programs for multiple generation production lines in accordance with institutional policies and procedures, including AEC and legislative requirements

select animals for breeding and apply appropriate breeding systems and mating methods and perform appropriate post-mating procedures in accordance with the breeding program and institutional standard operating procedures

identify and record animal type and genetic and strain names where appropriate

review, revise, maintain and update records in accordance with regulatory and project documentation requirements.

The skills and knowledge required to carry out advanced breeding procedures must be transferable to a range of work environments and contexts and include the ability to deal with unplanned events.

Context of and specific resources for assessment

Assessment of this unit is to be practical in nature and will be most appropriately assessed in an animal research facility or an environment that reproduces normal work conditions that has a scientific establishment licence and access to an approved AEC.

There must be access to a range of research animals and the relevant information, materials and documentation to enable one to demonstrate competence.

Method of assessment

To ensure consistency in one's performance, competency should be demonstrated, to industry defined standards, on more than two occasions over a period of time in order to cover a variety of circumstances, cases and responsibilities and over a number of assessment activities.

The assessment strategy must include practical skills assessment. Suggested strategies for this unit are:

written and/or oral assessment of candidate's required knowledge

observed, documented and first-hand testimonial evidence of candidate's application of practical tasks

practical simulation exercises that reproduce normal breeding work-related conditions

case study analysis

third-party evidence

workplace documentation

portfolio.

This unit may be assessed in a holistic way with other units of competency relevant to the industry sector, workplace and job role.

Guidance information for assessment

Assessment methods should reflect workplace demands (e.g. literacy and numeracy demands) and the needs of particular target groups (e.g. people with disabilities, Aboriginal and Torres Strait Islander people, women, people with a language background other than English, youth and people from low socioeconomic backgrounds).

Required Skills and Knowledge

Required skills

apply AEC classification system to determine procedures that require approval

check animals' physical conditions and vital signs and monitor for signs of progress or deterioration in condition or health of animals

employ safe and environmentally responsible organisational systems and procedures when handling animals, materials and equipment

identify health, congenital reproductive and parturition problems in breeding animals and respond in compliance with animal welfare and workplace project protocols

identify the sex, age and reproductive status of male and female animals

interpret and establish a breeding program consisting of multiple generations

maintain the highest standards of hygiene and infection control at all times to reduce the risk of infection and cross-infection

prepare and maintain appropriate records relevant to breeding and technical procedures using relevant institutional electronic and/or manual systems

prepare and use equipment and materials correctly and in accordance with manufacturers' specifications

prepare facilities and appropriate environments for breeding animals, embryo development, hatching or parturition and nursing animals

literacy skills to read, interpret and apply institution policies and procedures, including OHS, infection control, containment and exclusion and waste management; critically analyse material and record information collected accurately and legibly

oral communication skills/language to fulfil the job role as specified by the institution, including questioning, active listening, asking for clarification and consulting with or seeking advice from research group team members, senior or more experienced staff or other relevant persons

numeracy skills to estimate, calculate and record routine and more complex workplace measures relative to the establishing and designing of breeding procedures

interpersonal skills to work with others and relate to people from a range of cultural, social and religious backgrounds and with a range of physical and mental abilities

problem-solving skills to use available information and resources and carry out breeding procedures

use personal protective clothing and equipment correctly

use safe manual handling techniques and/or equipment

use safe waste handling and disposal procedures.

Required knowledge

anatomical and physiological structures and functions related to animal reproductive health and wellbeing of commonly held animals

anatomical and physiological terminology, and glossary of terms and nomenclature related to animal reproduction

artificial insemination procedures, including cryopreservation techniques

common diseases, ailments, injuries and other impacts on animal health and wellbeing and characteristics of healthy, sick or distressed animals

common pedigrees

effective knowledge of genetics including principles, common terminology and nomenclature

functions and requirements of a breeding colony

knockout and transgenic technology

organisational policies and safe operating procedures, including OHS and emergency procedures

oestrus cycles of a range of species, including representative polyoestrus, mono-oestrus and induced ovulation species

principles of animal welfare and ethics

principles of post-weaning management

range of mating systems, behaviour and methods appropriate to the species

recordkeeping requirements including types of information that needs to be kept on birthing and fostering processes

relevant codes of practice, such as the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes

relevant state or territory legislation and regulations relating to the practice of veterinary science, OHS and animal welfare and research, including the Office of the Gene Technology Regulator and the National Health and Medical Research Council (NHMRC)

reproductive cycles

safe animal handling techniques, including approved handling methods during mating and breeding programs

workplace hygiene standards, disinfectants, cleaning agents, cleaning techniques and cleaning equipment and materials.

Range Statement

The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included.

Institutional policies and procedures may include:

institution's quality assurance manual and procedures:

biosecurity

breeding processes

OHS

recycling and re-use guidelines

restraint, handling, euthanasing and disposal of animals

safe handling, storage and thawing of frozen genetic material

standard operating procedures

use, storage and transport of equipment and drugs used during procedures

waste disposal

project objectives and production schedules.

Relative legislative requirements may include:

Australian Code of Practice for the Care and Use of Animals for Scientific Purposes

Federal Gene Technology Act 2000

NHMRC guidelines

relevant state or territory legislation and regulations such as those relating to:

animal research

animal welfare

prevention of cruelty to animals

quarantine

the practice of veterinary surgery

the administration and storage of therapeutic and controlled substances.

OHS risks associated with animal technician procedures include:

animal bites, kicks or scratches

biological hazardous waste and sharps disposal

handling of chemicals and medicines

gas leakage

inhalation of aerosol particles

intraocular contamination

manual handling, including carrying, lifting and shifting

needle pricks and cuts from other sharps

release of infective agents (animal and human)

zoonoses.

Genetics may include:

genetic concepts:

advanced punnet squares

allele

autosomes

cell differentiation and genetic integrity

creation of transgenic, knock in and knockout lines

chromosomes and inheritance

coefficient of inbreeding

defining the differences between knock in, knock out, transgenic and genetic drift

genotype

heterozygosity

homozygosity

locus

nomenclature

phenotype

polygenic

recessive and recombinant genes

segregation

sex-linked genes.

Breeding program may include:

information about:

number of breeders required

selection criteria for breeders and retiring breeders

breeding system to be used:

backcross

inbred

outbred

mating system to be used

fostering requirements

identification system to be used

record systems to be used:

books

cards

computer

data to be collected about:

parent information

generation development (e.g. litter numbers, numbers born and sex ratios)

sex and weaning rates.

Subject to research requirements animals may include:

on most occasions the animal species will be common laboratory animals:

guinea pigs

mice

rabbits

rats

on some occasions animal species may include:

amphibians, fish and reptiles

dogs and cats

fish

invertebrates

livestock (e.g. sheep, cattle and pigs)

native wildlife (e.g. marsupials and birds)

primates

poultry.

animals may be housed in an experimental or teaching facility and typically, but not always, in a laboratory setting.

Stages of oestrus cycle may include:

induced ovulation species

mono-oestrus

representative polyoestrus.

Appropriate environment may consider:

the species involved

the specific requirements of the breeding program:

day or night cycle

humidity

seasonal effects

special dietary requirements

temperature.

Mating systems and methods may include:

mating systems:

breeding of mutant and transgenic strains

controlled outbreeding

cross breeding

hybrid breeding

inbreeding

mating methods:

artificial insemination

dominant mutant

hand mating

paired mating

stud mating

the harem system.

Post-weaning management may include:

phenotype care and the selection of future breeding animals or research stock based on:

genotyping

health or history

supplement feeding.

Breeding records should include:

breeding history

parents information

date of birth

numbers born

stillborn

missing or found dead

sexes born

number weaned

strain, genotype and phenotype information.

Types of animals that may be identified include:

chromosomal aberration

congenic

genetically modified

hybrid

inbred

recombinant.