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Evidence Guide: AHCSDT303 - Perform a fluorescence test on seeds

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

AHCSDT303 - Perform a fluorescence test on seeds

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare for a fluorescence test

  1. Identify seed to be tested and complete records
  2. Prepare working sample from submitted sample
  3. Prepare the required number of replicates of 100 seeds
  4. Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary
  5. Place replicates in a germination cabinet/room
Identify seed to be tested and complete records

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare working sample from submitted sample

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare the required number of replicates of 100 seeds

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Place replicates in a germination cabinet/room

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assess germinated seeds

  1. Count off and record all un-germinated seeds and abnormal seedlings
  2. Examine normal seedlings under ultra violet light
  3. Count off and record normal seedlings that have fluorescent roots under ultra violet light
  4. Count off and record normal seedlings that do not have fluorescent roots under ultra violet light
  5. Minimise exposure of bare skin to ultra violet light
  6. Complete enterprise worksheet
Count off and record all un-germinated seeds and abnormal seedlings

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine normal seedlings under ultra violet light

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Count off and record normal seedlings that have fluorescent roots under ultra violet light

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Count off and record normal seedlings that do not have fluorescent roots under ultra violet light

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Minimise exposure of bare skin to ultra violet light

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete enterprise worksheet

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Record, calculate and report results of test
  2. Collect and dispose of laboratory and hazardous waste safely
  3. Clean and store apparatus and equipment
Record, calculate and report results of test

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Collect and dispose of laboratory and hazardous waste safely

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Clean and store apparatus and equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Evidence Guide

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare for a fluorescence test

1.1 Identify seed to be tested and complete records

1.2 Prepare working sample from submitted sample

1.3 Prepare the required number of replicates of 100 seeds

1.4 Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary

1.5 Place replicates in a germination cabinet/room

2. Assess germinated seeds

2.1 Count off and record all un-germinated seeds and abnormal seedlings

2.2 Examine normal seedlings under ultra violet light

2.3 Count off and record normal seedlings that have fluorescent roots under ultra violet light

2.4 Count off and record normal seedlings that do not have fluorescent roots under ultra violet light

2.5 Minimise exposure of bare skin to ultra violet light

2.6 Complete enterprise worksheet

3. Maintain a safe work environment

3.1 Record, calculate and report results of test

3.2 Collect and dispose of laboratory and hazardous waste safely

3.3 Clean and store apparatus and equipment

Required Skills and Knowledge

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare for a fluorescence test

1.1 Identify seed to be tested and complete records

1.2 Prepare working sample from submitted sample

1.3 Prepare the required number of replicates of 100 seeds

1.4 Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary

1.5 Place replicates in a germination cabinet/room

2. Assess germinated seeds

2.1 Count off and record all un-germinated seeds and abnormal seedlings

2.2 Examine normal seedlings under ultra violet light

2.3 Count off and record normal seedlings that have fluorescent roots under ultra violet light

2.4 Count off and record normal seedlings that do not have fluorescent roots under ultra violet light

2.5 Minimise exposure of bare skin to ultra violet light

2.6 Complete enterprise worksheet

3. Maintain a safe work environment

3.1 Record, calculate and report results of test

3.2 Collect and dispose of laboratory and hazardous waste safely

3.3 Clean and store apparatus and equipment

The candidate must be assessed on their ability to integrate and apply the performance requirements of this unit in a workplace setting. Performance must be demonstrated consistently over time and in a suitable range of contexts.

The candidate must provide evidence that they can:

prepare for a fluorescence test

assess seedlings using ultra violet light

count and record test results

prepare replicates from working sample

test to determine the presence of seeds of annual ryegrass species in perennial ryegrass seedlots

maintain a safe work environment

The candidate must demonstrate knowledge of:

enterprise and/or legal traceability requirements

enterprise standard operating procedures (SOPs)

fluorescence testing procedures

relevant work health, safety and environment requirements

seed biology

the role of the International Seed Testing Association (ISTA) and the ISTA Rules for Seed Testing

Range Statement