Perform an endophytic seed test

Formats and tools

Unit Description
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Assessment Tool
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Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver AHCSDT407, 'Perform an endophytic seed test'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Books
Reference books for 'Perform an endophytic seed test' on fishpond.com.au. This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to ntisthis.com for every purchase, so go nuts :)


Elements and Performance Criteria

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare for endophyte testing

1.1 Review test request to identify samples to be tested, test methods and equipment

1.2 Clean and prepare equipment and working space

1.3 Prepare endophyte staining solution and alkaline or acid solution depending on method to be used

1.4 Prepare working sample from Pure Seed

2. Test seed for endophyte presence

2.1 Soften seed for recommended time in the prepared solution at the prescribed temperature

2.2 Rinse seed thoroughly in running tap water

2.3 Remove lemma, palea and endosperm from seed and place the remaining tissue on a microscope slide in a drop of endophyte staining solution crushing if necessary

2.4 Place cover glass on seed, applying gentle pressure and blot up excess stain

2.5 Examine seed with compound microscope and score as positive if endophytic hyphae are present, or negative if absent

3. Test seedlings for endophyte presence

3.1 Take 200-250 seeds from working sample at random

3.2 Sow seeds in good quality commercial potting mix at low density to allow for good growth

3.3 Examine well-developed seedlings from the germinated sample after growing for a minimum of 42 days

3.4 Cut seedling away from remnant seed and remove outermost sheath from the base of the seedling

3.5 Discard sheaths which are discoloured or have developed chlorophyll

3.6 Isolate a 3-5 mm wide longitudinal section of the sheath and place section on a microscope slide with the epidermis side down

3.7 Stain section immediately with the endophyte staining solution for prescribed time period

3.8 Place cover glass over the stained sheath section, press gently and blot of any excess staining solution

3.9 Examine the section with compound microscope and score as positive or negative if endophytic hyphae are present or absent

4. Record and report test results

4.1 Enter results under ‘other determinations’

4.2 Record sample size used for testing and whether seeds or seedlings were tested

4.3 Report results in terms of the percentage of seeds or seedlings in which endophyte was detected


Qualifications and Skillsets

AHCSDT407 appears in the following qualifications:

  • AHC41512 - Certificate IV in Seed Testing
  • AHC41516 - Certificate IV in Seed Testing