Perform an electrophoresis test on a seed sample

Formats and tools

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Assessment Tool
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Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
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Evidence Guide
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Competency Mapping Template
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Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver AHCSDT408, 'Perform an electrophoresis test on a seed sample'.
Google Links
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Books
Reference books for 'Perform an electrophoresis test on a seed sample' on fishpond.com.au. This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to ntisthis.com for every purchase, so go nuts :)


Elements and Performance Criteria

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare seed extraction and gel

1.1 Prepare working sample from submitted sample

1.2 Identify seed to be tested and complete records

1.3 Cut or crush single seeds and transfer to centrifuge tubes

1.4 Add extraction solution and mix with crushed seeds and allow to stand for prescribed period

1.5 Centrifuge tubes and collect and store supernatants

1.6 Prepare gel mixture and chemicals and use to achieve required level of polymerisation

2. Carry out electrophoresis

2.1 Place buffer solution in tanks

2.2 Load samples into wells and place gel in tanks

2.3 Carry out electrophoresis at the prescribed voltage and duration

2.4 Maintain temperature as required throughout the process

3. Fix and stain sample

3.1 Remove gel from tank and treat with chemicals as prescribed

3.2 Wash gel and treat to enhance stain

3.3 Examine and/or photograph stain

4. Evaluate stain

4.1 Compare banding pattern with that of an authentic variety or that of a known control sample

4.2 Provide records and reports

4.3 Determine if obvious procedure or equipment problems have led to atypical data or results

5. Maintain a safe work environment

5.1 Use established safe work practices and personal protective equipment

5.2 Minimise the generation of wastes and environmental impacts

5.3 Ensure the safe collection of laboratory and hazardous waste for subsequent disposal

5.4 Care for and store equipment and reagents

6. Maintain laboratory records

6.1 Enter approved data into laboratory information management system

6.2 Maintain confidentiality and security of enterprise information and laboratory data


Qualifications and Skillsets

AHCSDT408 appears in the following qualifications:

  • AHC41512 - Certificate IV in Seed Testing
  • AHC41516 - Certificate IV in Seed Testing