Perform an electrophoresis test on a seed sample

Formats and tools

Unit Description
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Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver AHCSDT408A, 'Perform an electrophoresis test on a seed sample'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Books
Reference books for 'Perform an electrophoresis test on a seed sample' on fishpond.com.au. This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to ntisthis.com for every purchase, so go nuts :)


Elements and Performance Criteria

1. Prepare seed extraction and gel

1.1 Working sample is prepared from submitted sample.

1.2 Seed to be tested is identified and records completed.

1.3 Single seeds are cut or crushed and transferred to centrifuge tubes.

1.4 Extraction solution is added and mixed with crushed seeds and allowed to stand for prescribed period.

1.5 Tubes are centrifuged and supernatants are collected and stored.

1.6 Gel mixture is prepared and chemicals and used to achieve required level of polymerisation.

2. Carry out electrophoresis

2.1 Buffer solution is placed in tanks.

2.2 Samples are loaded into wells and gel is placed in tanks.

2.3 Electrophoresis is carried out at the prescribed voltage and duration.

2.4 Temperature is maintained as required throughout the process

3. Fix and stain sample

3.1 Gel is removed from tank and treated with chemicals as prescribed.

3.2 Gel is washed and treated to enhance stain.

3.3 Stain is examined and/or photographed.

4. Evaluate stain

4.1 Banding pattern is compared with that of authentic variety or that of a known control sample.

4.2 Records and reports are provided in accordance with enterprise procedures.

4.3 Determine if obvious procedure or equipment problems have led to atypical data or results..

5. Maintain a safe work environment

5.1 Established safe work practices and personal protective equipment are used to ensure personal safety and that of other laboratory personnel.

5.2 The generation of wastes and environmental impacts are minimised.

5.3 The safe collection of laboratory and hazardous waste for subsequent disposal is ensured.

5.4 Equipment and reagents are cared for and stored as required

6. Maintain laboratory records

6.1 Approved data is entered into laboratory information management system.

6.2 Confidentiality and security of enterprise information and laboratory data is maintained.


Qualifications and Skillsets

AHCSDT408A appears in the following qualifications:

  • AHC41512 - Certificate IV in Seed Testing