Perform a tetrazolium test

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver AHCSDT409, 'Perform a tetrazolium test'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Reference books for 'Perform a tetrazolium test' on This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to for every purchase, so go nuts :)

Elements and Performance Criteria


Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare replicates for testing

1.1 Draw seeds from the pure seed fraction for the number of replicates to be tested ensuring there is no selection of seed which may cause biased results

1.2 Use safe work practices and personal protective equipment

2. Prepare seed and tetrazolium solution

2.1 Pre-moisten seed under prescribed temperature and light conditions to reduce possibility of injury to seeds when puncturing or cutting and to enable penetration of the tetrazolium solution

2.2 Remove sticky mucilage from seeds as necessary

2.3 Expose seed tissue before staining as appropriate for the species being tested

2.4 Keep seeds moist before testing

2.5 Prepare tetrazolium solution using distilled water and add trace amounts of fungicide or antibiotics to solution if necessary for the species being tested

3. Stain with tetrazolium solution

3.1 Immerse seeds in tetrazolium solution

3.2 Maintain test at a temperature within the range 20 to 40°C, and maintain low light levels to facilitate staining

3.3 Increase duration of staining if temperatures below 30°C are used or decrease if higher temperatures are used

3.4 Decant the solution at end of staining period, rinse seeds with water and keep damp

4. Evaluate test results

4.1 Examine and evaluate each seed as viable or non-viable based on staining patterns applicable to the species being tested

4.2 Count the number of seeds considered viable in each replicate

4.3 Calculate the percentage of viable seed in each replicate and determine average percentage of viable seed across all replicates

5. Finalise reporting and clean up

5.1 Record and report results of test

5.2 Collect and dispose of laboratory and hazardous waste

5.3 Clean apparatus and equipment used in tetrazolium test and dispose of used chemicals and solutions safely