Perform a tetrazolium seed viability test

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver AHCSDT409A, 'Perform a tetrazolium seed viability test'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Books
Reference books for 'Perform a tetrazolium seed viability test' on fishpond.com.au. This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to ntisthis.com for every purchase, so go nuts :)


Elements and Performance Criteria

1. Prepare replicates for testing

1.1 Pure Seed is mixed thoroughly to reduce bias in results.

1.2 Prescribed number of replicates are drawn from the Pure Seed fraction according to enterprise guidelines.

1.3 Safe work practices and personal protective equipment are used to ensure personal safety and that of other laboratory personnel.

2. Pre-moisten replicates

2.1 Seed is pre-moistened to reduce possibility of injury to seeds when puncturing or cutting and to enable penetration of the tetrazolium solution under prescribed temperature and light conditions.

2.2 Sticky mucilage is removed from seeds as necessary.

2.3 Seed tissue is exposed prior to staining according to enterprise guidelines.

2.4 Seeds are kept moist prior to testing.

3. Stain with tetrazolium solution

3.1 Seeds are completely immersed in tetrazolium solution according to enterprise guidelines.

3.2 Fungicides and/or antibiotics may be added to solution according to enterprise guidelines.

3.3 Low light levels are maintained as required by testing procedures to facilitate staining.

3.4 At completion of staining period, the solution is decanted and seeds are rinsed with water.

4. Evaluate test results

4.1 Each seed is examined and evaluated as viable or non-viable based on staining patterns applicable to the species being tested.

4.2 The number of seeds considered viable in each replicate is counted.

4.3 The percentage of viable seed in each replicate is calculated and average percentage of viable seed across all replicates is determined.

5. Finalise reporting and clean up

5.1 Results of test are recorded and reported according to enterprise guidelines.

5.2 Laboratory and hazardous waste are safely handled, collected and disposed of according to enterprise guidelines.

5.3 Apparatus and equipment used in tetrazolium test is cleaned and used chemicals and solutions are disposed of safely in accordance with enterprise guidelines.


Qualifications and Skillsets

AHCSDT409A appears in the following qualifications:

  • AHC41512 - Certificate IV in Seed Testing