Prepare culture media

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL973003A, 'Prepare culture media'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Reference books for 'Prepare culture media' on This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to for every purchase, so go nuts :)

Elements and Performance Criteria



1. Prepare culture media

1.1. Prepare mixture of media and solvent to ensure solution and even settling of heat soluble materials

1.2. Label media to allow tracking in subsequent processes

1.3. Use a vessel large enough to endure adequate mixing and heating of the media

1.4. Dispense media into vessels for sterilisation, leaving room for expansion during heating and cooling

2. Sterilise media

2.1. Load the steriliser in keeping with maximum permitted loads and appropriate positioning of materials

2.2. Ensure a sterilisation indicator is correctly placed with the load to monitor sterilisation process

2.3. Operate sterilisation cycle in accordance with manufacturer's requirements to achieve sterilisation at the required settings

2.4. Cool media to the temperature specified in the media formulation procedures

3. Pour, label and store media

3.1. Add labile constituents where necessary, under conditions that will not lead to their denaturation or contamination of media

3.2. Ensure even mixing of additives and media before dispensing

3.3. Aseptically dispense media to minimise occurrence of procedural contamination

3.4. Label media to allow for selection, avoiding areas of the culture vessel required for examination of colony growth

3.5. Store media to maximise shelf life and minimise contamination

3.6. Date batch media to ensure correct batch rotation

3.7. Incubate control plates as a sterility check

4. Perform quality control checks

4.1. Inspect media for any evidence of possible contamination or problems with structure or sterilisation

4.2. Check useability of selective media by growth of expected organism

4.3. Check stored stocks at regular intervals for conformance to required standards

5. Maintain work area and equipment to prevent cross-infection and contamination

5.1. Use personal protective equipment and safe work practices to ensure safety of self and others

5.2. Place disposable and reusable items into relevant receptacles

5.3. Clean and disinfect work area and equipment after use

5.4. Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

Qualifications and Skillsets

MSL973003A appears in the following qualifications:

  • MSA40108 - Certificate IV in Manufacturing Technology
  • MSA30208 - Certificate III in Manufacturing Technology
  • PSP30604 - Certificate III in Government (School Support Services)
  • PSP30704 - Certificate III in School Support Services
  • MTM30611 - Certificate III in Meat Processing (General)
  • HLT32607 - Certificate III in Pathology
  • MSL50109 - Diploma of Laboratory Technology
  • MSL20109 - Certificate II in Sampling and Measurement
  • MSL30109 - Certificate III in Laboratory Skills
  • MSL40109 - Certificate IV in Laboratory Techniques

And the skillsets: