Perform histological procedures

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL973008A, 'Perform histological procedures'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Reference books for 'Perform histological procedures' on This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to for every purchase, so go nuts :)

Elements and Performance Criteria



1. Assemble equipment and materials

1.1. Confirm the number and type of sections required

1.2. Collect equipment and arrange the workspace so that equipment can be used safely and efficiently

1.3. Perform pre-use and safety checks to ensure equipment is fit for purpose

1.4. Report faulty or unsafe equipment to appropriate personnel

1.5. Inspect processor reagents for deterioration and adequate volume and report any items requiring replacement

2. Process and embed plant and animal tissue

2.1. Select program and reagents for processing

2.2. Monitor processor regularly during processing sequence to ensure dehydration, clearing and infiltration are complete

2.3. Check that temperature of wax is suitable for embedding process

2.4. Check that volume of wax is sufficient for uninterrupted embedding of processor load

2.5. Embed tissue in correct orientation

2.6. Allow block to solidify evenly according to wax requirements

3. Cut sections of plant and animal tissue

3.1. Place and secure block and knife in microtome strictly in accordance with safety directions

3.2. Label required number of microscope slides in accordance with enterprise traceability requirements

3.3. Cut ribbons of representative sections at the required thickness observing prescribed safety measures

3.4. Float sections onto water bath to flatten tissues

3.5. Pick up sections onto microscope slides ensuring identification on slides matches that on block

3.6. Apply procedures to prevent cross-contamination between samples

3.7. Cut free hand sections of plant tissue as required

3.8. Inspect sections and reject items that do not meet specifications

4. Stain sections

4.1. Select reagents specified in the method

4.2. Stain sections according to the method

4.3. Examine sections microscopically to ensure expected staining outcomes have been achieved

4.4. Mount sections to ensure long term preservation

4.5. Attach permanent labels giving specimen details according to enterprise traceability requirements

5. Maintain a safe work environment

5.1. Ensure personal safety and minimise cross-contamination through the use of personal protective equipment

5.2. Handle all specimens and equipment in accordance with enterprise safety protocols/procedures

5.3. Clean up spills using appropriate techniques to protect personnel, work area and environment

5.4. Minimise generation of waste and environmental impacts

5.5. Collect and dispose of all wastes safely

5.6. Report hazards and incidents to designated personnel using enterprise procedures

Qualifications and Skillsets

MSL973008A appears in the following qualifications:

  • ACM30210 - Certificate III in Animal Technology
  • MSL50109 - Diploma of Laboratory Technology
  • MSL20109 - Certificate II in Sampling and Measurement
  • MSL30109 - Certificate III in Laboratory Skills
  • MSL40109 - Certificate IV in Laboratory Techniques