Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Receive samples and process associated request forms

1.1

Check samples and request form details before they are accepted

1.2

Return samples and request forms that do not comply with requirements to source with reasons for non-acceptance

1.3

Log samples, recording details that allow accurate tracking and chain of custody

1.4

Distribute samples for local testing or dispatch samples to other testing facilities

1.5

Store samples appropriately where testing or transport is to be delayed

2

Prepare for safe microbiological work and aseptic applications

2.1

Select work area and equipment required for the safe handling of materials that may contain microorganisms of specified risk groups

2.2

Wear protective apparel, replacing it when contamination is suspected

2.3

Apply correct disinfection procedures to work areas before and after use

2.4

Locate relevant emergency equipment for timely response to microbiological accidents

2.5

Apply standard precautions when handling biological materials

2.6

Minimise the production and release of aerosols, using biological safety cabinets where necessary

2.7

Clean spills, and report all spills and suspected incidents to supervisor

2.8

Wash hands before and after laboratory work and when contamination is suspected

2.9

Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with workplace procedures

3

Process samples for direct examination

3.1

Prepare thin smears of samples for subsequent staining to enable microscopic identification of cells

3.2

Prepare liquid films of specimens for direct observation for motility or cell structure

3.3

Prepare samples to concentrate material for subsequent staining or microscopy

4

Prepare pure cultures for microbiological work and aseptic applications

4.1

Select culture media to maximise growth of microorganisms and cells

4.2

Inoculate media aseptically, applying techniques suitable for purpose of culture

4.3

Incubate inoculated media in conditions to optimise growth of organisms and cells

4.4

Subculture on suitable media to optimise production of pure cultures

5

Perform procedures that can assist in the identification of microorganisms

5.1

Select staining techniques to demonstrate required cellular characteristics

5.2

Stain prepared films to demonstrate diagnostically useful characteristics

5.3

Inoculate and incubate media with pure cultures to assist in the biochemical and immunological identification of microorganisms

5.4

Perform tests on pure cultures to assist in the biochemical and immunological identification of microorganisms

5.5

Perform antibiotic sensitivity testing, if required

6

Estimate the number and/or size of micro-organisms in samples

6.1

Count cells in undiluted samples to indicate the dilution necessary to reliably count organisms in culture

6.2

Prepare serial dilutions of samples aseptically for culture and colony counting

6.3

Count colonies for calculating number of viable organisms per unit volume

6.4

Count microorganisms in samples and cultures using spectrometric and electronic methodologies, where relevant

6.5

Estimate and document uncertainty of measurement in accordance with workplace procedures, where relevant

7

Maintain records of laboratory work

7.1

Make entries on report forms or into computer systems, accurately calculating, recording or transcribing data as required

7.2

Maintain instrument logs as required by accreditation checklists

7.3

Maintain security and confidentiality of all clinical information, laboratory data and records