Perform microbiological tests

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL975001A, 'Perform microbiological tests'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Reference books for 'Perform microbiological tests' on This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to for every purchase, so go nuts :)

Elements and Performance Criteria



1. Receive samples and process associated request forms

1.1. Check samples and request form details before they are accepted

1.2. Return samples and request forms that do not comply with requirements to source with reasons for non-acceptance

1.3. Log samples, recording details that allow accurate tracking and chain of custody

1.4. Distribute samples for local testing or dispatch samples to other testing facilities

1.5. Store samples appropriately where testing or transport is to be delayed

2. Prepare for safe microbiological work and aseptic applications

2.1. Select work area and equipment required for the safe handling of materials that may contain micro-organisms of specified risk groups

2.2. Wear protective apparel, replacing it when contamination is suspected

2.3. Apply correct disinfection procedures to work areas before and after use

2.4. Locate relevant emergency equipment for timely response to microbiological accidents

2.5. Apply standard precautions when handling biological materials

2.6. Minimise the production and release of aerosols, using biological safety cabinets where necessary

2.7. Clean spills, and report all spills and suspected incidents to supervisor

2.8. Wash hands before and after laboratory work and when contamination is suspected

2.9. Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with enterprise procedures

3. Process samples for direct examination

3.1. Prepare thin smears of samples for subsequent staining to enable microscopic identification of cells

3.2. Prepare liquid films of specimens for direct observation for motility or cell structure

3.3. Prepare samples to concentrate material for subsequent staining or microscopy

4. Prepare pure cultures for microbiological work and aseptic applications

4.1. Select culture media to maximise growth of micro-organisms and cells

4.2. Inoculate media aseptically, applying techniques suitable for purpose of culture

4.3. Incubate inoculated media in conditions to optimise growth of organisms and cells

4.4. Subculture on suitable media to optimise production of pure cultures

5. Perform procedures that can assist in the identification of micro-organisms

5.1. Select staining techniques to demonstrate required cellular characteristics

5.2. Stain prepared films to demonstrate diagnostically useful characteristics

5.3. Inoculate and incubate media with pure cultures to assist in the biochemical and immunological identification of micro-organisms

5.4. Perform tests on pure cultures to assist in the biochemical and immunological identification of micro-organisms

6. Estimate the number and/or size of micro-organisms in samples

6.1. Count cells in undiluted samples to indicate the dilution necessary to reliably count organisms in culture

6.2. Prepare serial dilutions of samples aseptically for culture and colony counting

6.3. Count colonies for calculating number of viable organisms per unit volume

6.4. Count micro-organisms in samples and cultures using spectrometric and electronic methodologies, where relevant

6.5. Estimate and document uncertainty of measurement in accordance with enterprise procedures, where relevant

7. Contribute to antibiotic sensitivity testing where required

7.1. Prepare inoculum suitable for antibiotic sensitivity testing

7.2. Dispense or position antibiotic discs as indicated by enterprise protocol

7.3. Incubate inoculated media under conditions to maximise growth of cultured organism

7.4. Read and record sensitivity reactions, noting phenomena that can assist in the correct interpretation of results

8. Maintain records of laboratory work

8.1. Make entries on report forms or into computer systems, accurately calculating, recording or transcribing data as required

8.2. Maintain instrument logs as required by accreditation checklists

8.3. Maintain security and confidentiality of all clinical information, laboratory data and records