Perform histological tests

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL975003, 'Perform histological tests'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Books
Reference books for 'Perform histological tests' on fishpond.com.au. This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to ntisthis.com for every purchase, so go nuts :)


Elements and Performance Criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Process specimens and associated request forms

1.1

Check and match specimens and request forms before they are accepted

1.2

Identify specimens and request forms that do not comply with requirements, record any discrepancies and indicate what action is required

1.3

Log acceptable specimens into a laboratory information management system (LIMS) accurately and efficiently, applying required document tracking mechanisms

1.4

Store specimens appropriately until required for testing

2

Prepare specimens for cut-up

2.1

Arrange tissues and request forms in cut-up area

2.2

Label tissue cassettes as required to maintain identity during subsequent procedures

2.3

Select tissue fixative to prepare tissue for subsequent procedures

2.4

Weigh organs and count tissue chips and shavings

3

Process tissue

3.1

Select processor program and reagents

3.2

Perform equipment pre-use checks on the tissue processor to ensure sample integrity is maintained

3.3

Follow processing requirements for non-routine techniques, including histochemistry

4

Embed tissue

4.1

Check that temperature of embedding medium is suitable for embedding process

4.2

Check that volume of embedding medium is sufficient for uninterrupted embedding of processor load

4.3

Embed tissue types in correct orientation and characteristics that minimise tissue loss during microtomy

4.4

Apply procedures to prevent cross-contamination between tissues

4.5

Inspect blocks, reject and re-embed items that do not meet quality control standards

5

Cut tissue sections

5.1

Check that flotation bath is ready and satisfactory for use

5.2

Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch

5.3

Secure block in microtome following specified safety directions

5.4

Orientate the block correctly for each specific tissue to ensure tissue conservation and maintain sample integrity

5.5

Label required number of microscope slides in accordance with workplace traceability requirements

5.6

Cut thin tissue sections (4-5μm), according to needs of subsequent procedures, maintaining sample integrity and conserving tissue for further testing

5.7

Float sections onto water bath to flatten tissues

5.8

Pick up sections onto microscope slides ensuring patient identification on slides matches that on block

5.9

Apply procedures to prevent cross-contamination between patient tissues

5.10

Maintain tissue sections in conditions compatible with intended subsequent procedures

5.11

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

5.12

Inspect sections and reject any that do not meet quality control standards

513

Identify, troubleshoot and resolve common section quality control issues

6

Stain tissue sections

6.1

Apply staining procedures to demonstrate required morphological features

6.2

Prepare labile reagents for immediate use

6.3

Select reagents for specified technique, ensuring reagent sequence matches standard procedure

6.4

Stain sections according to method using the required quality control section and accommodating any authorised variations

6.5

Mount slides using medium compatible with staining technique

6.6

Examine control sections microscopically to ensure expected staining outcomes are achieved and procedural artefacts are detected

6.7

Confirm macroscopically or microscopically that tissues on all slides conform with the expected characteristics of that case

6.8

Participate in final check to establish that the number of slides tallies with the worksheet

6.9

Attach permanent label giving specimen details as required by workplace

6.10

Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain

7

Maintain a safe environment

7.1

Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

7.2

Handle non-fixed tissues safely to minimise cross-infection and contamination of personnel and environment

7.3

Store fixed tissues as specified to minimise exposure of personnel to dangerous fumes and vapours

7.4

Clean up spills using appropriate techniques to protect personnel, work area and environment from contamination

7.5

Minimise the generation of wastes

7.6

Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with workplace procedures

8

Maintain laboratory records

8.1

Make entries on report forms or into computer systems, accurately calculating, recording or transcribing data as required

8.2

Complete control stain quality assessment forms to report quality control outcomes

8.3

File and store tissue sections to facilitate efficient retrieval as required

8.4

Maintain instrument logs as required by accreditation checks

8.5

Maintain confidentiality and security of all clinical information, and laboratory data and records


Qualifications and Skillsets

MSL975003 appears in the following qualifications:

  • HLT51907 - Diploma of Mortuary Theatre Practice
  • HLT41807 - Certificate IV in Pathology
  • MSL50109 - Diploma of Laboratory Technology
  • MSL40109 - Certificate IV in Laboratory Techniques
  • MSL60109 - Advanced Diploma of Laboratory Operations
  • HLT51912 - Diploma of Mortuary Theatre Practice
  • HLT41812 - Certificate IV in Pathology
  • MSL60116 - Advanced Diploma of Laboratory Operations
  • MSL40116 - Certificate IV in Laboratory Techniques
  • MSL50116 - Diploma of Laboratory Technology