Perform histological tests

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL975003A, 'Perform histological tests'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Reference books for 'Perform histological tests' on This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to for every purchase, so go nuts :)

Elements and Performance Criteria



1. Process specimens and associated request forms

1.1. Check and match specimens and request forms before they are accepted

1.2. Return specimens and request forms that do not comply with requirements to their source with reasons for non-acceptance

1.3. Process routine and non-routine specimens according to enterprise protocols

1.4. Log acceptable specimens, applying required document tracking mechanisms

1.5. Dispatch specimens to referral laboratories as required

1.6. Store specimens appropriately until required for testing

2. Prepare specimens for cut-up

2.1. Arrange tissues and request forms in cut-up area

2.2. Label tissue cassettes as required to maintain identity during subsequent procedures

2.3. Prepare containers for transport of tissues to processor

2.4. Select tissue fixative to prepare tissue for subsequent procedures

2.5. Weigh organs and count tissue chips and shavings

2.6. Take notes of gross features of specimens during cut-up if required

3. Process tissue

3.1. Select processor program and reagents

3.2. Inspect processor reagents for deterioration and adequate volume

3.3. Follow processing requirements for non-routine techniques, including histochemistry

3.4. Monitor processor regularly during processing sequence where appropriate

4. Embed tissue

4.1. Select embedding medium that is compatible with infiltrating agent

4.2. Check that temperature of embedding medium is suitable for embedding process

4.3. Check that volume of embedding medium is sufficient for uninterrupted embedding of processor load

4.4. Embed tissue in correct orientation

4.5. Apply procedures to prevent cross-contamination between patient tissues

4.6. Allow block to solidify according to requirements of embedding medium

5. Cut tissue sections

5.1. Check that flotation bath is ready and satisfactory for use

5.2. Prepare microtome and associated equipment to accommodate requirements of tissue batch

5.3. Secure block in microtome following specified safety procedures

5.4. Label required number of microscope slides with patient identification as prescribed by enterprise

5.5. Cut tissue sections according to needs of subsequent procedures

5.6. Float sections onto water bath to flatten tissues

5.7. Pick up sections onto microscope slides ensuring patient identification on slides matches that on block

5.8. Apply procedures to prevent cross-contamination between patient tissues

5.9. Maintain tissue sections in conditions compatible with intended subsequent procedures

6. Stain tissue sections

6.1. Apply staining procedures to demonstrate required morphological features

6.2. Prepare labile reagents for immediate use

6.3. Select reagents for specified technique, ensuring reagent sequence matches standard procedure

6.4. Stain sections according to method accommodating any authorised variations and applying required quality control

6.5. Mount slides using medium compatible with staining technique

6.6. Examine sections microscopically to ensure expected staining outcomes are achieved and procedural artefacts are detected

6.7. Confirm macroscopically or microscopically that tissue type conforms with labelling and pathologist specifications

6.8. Participate in final check to establish that the number of slides tallies with the worksheet

6.9. Attach permanent label giving specimen details as required by enterprise

7. Contribute to efficient provision of histological services

7.1. Monitor and maintain resources for pathologists in cut-up area

7.2. Liaise with clinical and nursing staff if required by enterprise regarding tissue fixative requirements in areas, such as wards, theatres and mortuary

7.3. Monitor and maintain volumes of fixatives in areas, such as wards, theatres and mortuary

7.4. Store slides and blocks according to legal and enterprise requirements under conditions that prevent degeneration

8. Maintain a safe environment

8.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel

8.2. Handle non-fixed tissues safely to minimise cross-infection and contamination of personnel and environment

8.3. Store fixed tissues as specified to minimise exposure of personnel to dangerous fumes and vapours

8.4. Clean up spills using appropriate techniques to protect personnel, work area and environment from contamination

8.5. Minimise the generation of wastes

8.6. Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with enterprise procedures

9. Maintain laboratory records

9.1. Make entries on report forms or into computer systems, accurately calculating, recording or transcribing data as required

9.2. File and store tissue sections to facilitate efficient retrieval as required

9.3. Maintain instrument logs as required by accreditation checks

9.4. Maintain confidentiality and security of all clinical information, and laboratory data and records

Qualifications and Skillsets

MSL975003A appears in the following qualifications:

  • HLT51907 - Diploma of Mortuary Theatre Practice
  • HLT41807 - Certificate IV in Pathology
  • MSL50109 - Diploma of Laboratory Technology
  • MSL40109 - Certificate IV in Laboratory Techniques
  • MSL60109 - Advanced Diploma of Laboratory Operations