Perform tissue and cell culture techniques

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL975013A, 'Perform tissue and cell culture techniques'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Reference books for 'Perform tissue and cell culture techniques' on This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to for every purchase, so go nuts :)

Elements and Performance Criteria



1. Interpret and schedule production requirements

1.1. Review client request and confirm quantity and nature of cells, tissue or products

1.2. Select, appropriate media, materials, equipment and methods

1.3. Plan parallel work sequences to optimise production

1.4. Maintain a chain of custody, traceable to the worker, for all cells and tissues

2. Work safely according to the legal and regulatory framework

2.1. Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures

2.2. Identify hazards and enterprise control measures associated with the sample, preparation methods, reagents and equipment

2.3. Select, fit and use personal protective clothing and safety equipment

2.4. Address hazards and incidents as they arise

2.5. Ensure the safe disposal of biohazardous materials and other laboratory wastes

3. Assemble and maintain tissue culture equipment

3.1. Assemble, sterilise or decontaminate equipment according to enterprise procedures

3.2. Perform pre-use and safety checks in accordance with relevant enterprise and operating procedures

3.3. Identify faulty or unsafe components and equipment and report to appropriate personnel

3.4. Decontaminate area and equipment after use

4. Prepare and test cell and tissue culture media

4.1. Confirm media specifications and processes/methods

4.2. Prepare culture media to suit client request

4.3. Sterilise culture media and check for sterility

4.4. Perform quality control checks to ensure that culture media is fit for purpose

4.5. Store culture media in accordance with specifications

5. Obtain, monitor and maintain tissue and cell lines

5.1. Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture

5.2. Select specified culture media and add any necessary growth agents or nutrients

5.3. Incubate cells/tissue in specified conditions

5.4. Inoculate the media with the specified amount of sample

5.5. Monitor growth of tissue and cell lines and products

5.6. Detect contamination and troubleshoot materials, equipment and techniques

5.7. Passage samples by subculturing to preserve or grow the line

5.8. Harvest cells or cell products to optimise yields

6. Preserve cells and tissues

6.1. Select the appropriate preservation method

6.2. Preserve the cell lines or tissue in accordance with the method

6.3. Check preserved cell lines regularly to ensure viability is maintained

7. Maintain records

7.1. Maintain records of batches of media and test data

7.2. Maintain records of active and stored tissue and cell lines

7.3. Ensure records are retrievable, legible and accurate

7.4. Ensure records conform to the information management, records, quality system and legal requirements

Qualifications and Skillsets

MSL975013A appears in the following qualifications:

  • HLT41807 - Certificate IV in Pathology
  • MSL50109 - Diploma of Laboratory Technology
  • MSL40109 - Certificate IV in Laboratory Techniques
  • MSL60109 - Advanced Diploma of Laboratory Operations