Apply specialised knowledge of liquid chromatography techniques to analysis

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL977007A, 'Apply specialised knowledge of liquid chromatography techniques to analysis'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Reference books for 'Apply specialised knowledge of liquid chromatography techniques to analysis' on This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to for every purchase, so go nuts :)

Elements and Performance Criteria



1. Determine sample characteristics and appropriate analytical methods

1.1. Interpret client request and/or perform presumptive tests to identify sample characteristics that may affect analysis

1.2. Liaise with client or sample provider to review client needs, testing requirements and sample history, if necessary

1.3. Identify analytical standards, reference materials, test methods and enterprise procedures that may be applicable

1.4. Select the most appropriate standard test method that is consistent with testing requirements and instrument availability

1.5. If no standard method exists, adapt or modify a test method to suit the sample characteristics

1.6. If necessary, seek advice from supervisor about any proposed variations and document all approved changes to test methods

1.7. Schedule analysis using enterprise procedures

2. Prepare samples, standards and mobile phase

2.1. Log sample into instrument software

2.2. Obtain a representative analytical portion of the laboratory sample

2.3. Prepare sample in accordance with selected test method

2.4. Prepare mobile phase in accordance with selected test method using buffers and other additives to enhance separation and ionisation, concentrate analytes and prevent build up of salts, as necessary

2.5. Filter and degas mobile phase

2.6. Prepare validation checks and/or calibration standards for analytical portions

2.7. Use specialised procedures for ultra trace sample and standard preparation as required

3. Set up instrument and perform trial analysis

3.1. Configure the injector, column, solvent delivery and detector sub-systems according to the selected test method

3.2. Prime the pump, flush the system and monitor pressures and baseline to ensure the system is fully equilibrated

3.3. Perform other pre-use, calibration and safety checks using enterprise procedures

3.4. Set instrumental parameters in accordance with those specified in selected test method

3.5. Check and optimise each instrument sub-system

3.6. Conduct performance tests using standards and samples

3.7. Assess instrument performance in terms of response, resolution and run-time

4. Optimise instrument performance

4.1. Apply an understanding of analyte and column chemistry, temperature control and varying mobile phase composition to determine strategies for enhancing separation and detection of required species

4.2. Adjust instrumental parameters in a logical and efficient sequence to optimise performance

4.3. When optimum separation is achieved, check that the detector and system software can correctly identify and quantify the required species

5. Perform analysis

5.1. Measure analyte response for standards, validation checks and samples using optimised instrument settings

5.2. Conduct sufficient measurements to obtain reliable data

5.3. Use system software to produce calibration graphs, chromatographs and/or mass spectra, confirm data quality and calculate uncertainties

5.4. Check that results are consistent with estimations and expectations

5.5. Analyse trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel

5.6. Return instrument to standby or shutdown condition in accordance with enterprise procedures

5.7. Report results with the appropriate accuracy, precision, uncertainty and units

6. Perform routine maintenance and troubleshoot instruments

6.1. Flush and store the column with the recommended solvent to protect the stationary phase during downtime

6.2. Regularly check that the pump, lines, valves, injector, column and detector sub-systems are clean/undamaged and replace consumable items as necessary

6.3. Change columns and detectors in accordance with manufacturer's instructions and ensure that the system is free of leaks and properly conditioned before re-use

6.4. Investigate possible causes for the absence of peaks and presence of ghost peaks, split/distorted peaks, band broadening and apply recommended remedial actions

6.5. Investigate possible causes for baseline instability and non-reproducible retention times and apply recommended remedial actions

6.6. Identify the need for repairs or servicing and determine whether local repair/maintenance is technically possible and economic

6.7. Arrange for repair or servicing from an accredited agent or other appropriate personnel in accordance with enterprise procedures

7. Maintain a safe work environment

7.1. Identify risks, hazards, safety equipment and control measures associated with sample handling/preparation and test method

7.2. Use personal protective equipment and safety procedures specified for test method and materials to be tested

7.3. Minimise the generation of wastes and environmental impacts

7.4. Ensure the safe collection/disposal of mobile phase and other laboratory wastes

7.5. Clean, care for and store equipment and consumables in accordance with enterprise procedures

8. Maintain laboratory records

8.1. Enter approved data and results into laboratory information management system (LIMS)

8.2. Maintain logs of instrument calibration checks, use and maintenance in accordance with enterprise procedures

8.3. Maintain security, integrity and traceability of samples, results and documentation

8.4. Communicate results to appropriate personnel in accordance with enterprise procedures

Qualifications and Skillsets

MSL977007A appears in the following qualifications:

  • MSL70109 - Vocational Graduate Certificate in Instrumental Analysis