ELEMENT

PERFORMANCE CRITERIA

1. Determine sample characteristics and appropriate analytical methods

1.1. Interpret client request and/or perform presumptive tests to identify sample characteristics that may affect analysis

1.2. Liaise with client or sample provider to review client needs, testing requirements and sample history, if necessary

1.3. Identify analytical standards, reference materials, test methods and enterprise procedures that may be applicable

1.4. Select the most appropriate standard test method that is consistent with testing requirements and instrument availability

1.5. If no standard method exists, adapt or modify a test method to suit the sample characteristics

1.6. If necessary, seek advice from supervisor about any proposed variations and document all approved changes to test methods

1.7. Schedule analysis using enterprise procedures

2. Prepare samples, standards and mobile phase

2.1. Log sample into instrument software

2.2. Obtain a representative analytical portion of the laboratory sample

2.3. Prepare sample in accordance with selected test method

2.4. Prepare mobile phase in accordance with selected test method using buffers and other additives to enhance separation and ionisation, concentrate analytes and prevent build up of salts, as necessary

2.5. Filter and degas mobile phase

2.6. Prepare validation checks and/or calibration standards for analytical portions

2.7. Use specialised procedures for ultra trace sample and standard preparation as required

3. Set up instrument and perform trial analysis

3.1. Configure the injector, column, solvent delivery and detector sub-systems according to the selected test method

3.2. Prime the pump, flush the system and monitor pressures and baseline to ensure the system is fully equilibrated

3.3. Perform other pre-use, calibration and safety checks using enterprise procedures

3.4. Set instrumental parameters in accordance with those specified in selected test method

3.5. Check and optimise each instrument sub-system

3.6. Conduct performance tests using standards and samples

3.7. Assess instrument performance in terms of response, resolution and run-time

4. Optimise instrument performance

4.1. Apply an understanding of analyte and column chemistry, temperature control and varying mobile phase composition to determine strategies for enhancing separation and detection of required species

4.2. Adjust instrumental parameters in a logical and efficient sequence to optimise performance

4.3. When optimum separation is achieved, check that the detector and system software can correctly identify and quantify the required species

5. Perform analysis

5.1. Measure analyte response for standards, validation checks and samples using optimised instrument settings

5.2. Conduct sufficient measurements to obtain reliable data

5.3. Use system software to produce calibration graphs, chromatographs and/or mass spectra, confirm data quality and calculate uncertainties

5.4. Check that results are consistent with estimations and expectations

5.5. Analyse trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel

5.6. Return instrument to standby or shutdown condition in accordance with enterprise procedures

5.7. Report results with the appropriate accuracy, precision, uncertainty and units

6. Perform routine maintenance and troubleshoot instruments

6.1. Flush and store the column with the recommended solvent to protect the stationary phase during downtime

6.2. Regularly check that the pump, lines, valves, injector, column and detector sub-systems are clean/undamaged and replace consumable items as necessary

6.3. Change columns and detectors in accordance with manufacturer's instructions and ensure that the system is free of leaks and properly conditioned before re-use

6.4. Investigate possible causes for the absence of peaks and presence of ghost peaks, split/distorted peaks, band broadening and apply recommended remedial actions

6.5. Investigate possible causes for baseline instability and non-reproducible retention times and apply recommended remedial actions

6.6. Identify the need for repairs or servicing and determine whether local repair/maintenance is technically possible and economic

6.7. Arrange for repair or servicing from an accredited agent or other appropriate personnel in accordance with enterprise procedures

7. Maintain a safe work environment

7.1. Identify risks, hazards, safety equipment and control measures associated with sample handling/preparation and test method

7.2. Use personal protective equipment and safety procedures specified for test method and materials to be tested

7.3. Minimise the generation of wastes and environmental impacts

7.4. Ensure the safe collection/disposal of mobile phase and other laboratory wastes

7.5. Clean, care for and store equipment and consumables in accordance with enterprise procedures

8. Maintain laboratory records

8.1. Enter approved data and results into laboratory information management system (LIMS)

8.2. Maintain logs of instrument calibration checks, use and maintenance in accordance with enterprise procedures

8.3. Maintain security, integrity and traceability of samples, results and documentation

8.4. Communicate results to appropriate personnel in accordance with enterprise procedures