- MSL975014A - Perform molecular biology tests and procedures
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners
MSL975014A Mapping and Delivery Guide
Perform molecular biology tests and procedures
Version 1.0
Issue Date: April 2024
Qualification | - |
Unit of Competency | MSL975014A - Perform molecular biology tests and procedures |
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Description | This unit of competency covers the ability to isolate, purify, verify and manipulate biomolecules and their products. This work requires close attention to working with small volumes, multiple-step procedures and prevention of contamination. Personnel are required to apply a wide range of molecular biology tests and procedures. | ||
Employability Skills | This unit contains employability skills. | ||
Learning Outcomes and Application | This unit of competency is applicable to technical officers working in manufacturing (e.g. macro, micro, nanotechnology, pharmaceutical and blood product), food processing, biomedical (e.g. forensics, pathology and veterinary) and environmental industry sectors. All operations must comply with relevant regulations, codes of practice, standards, test methods and enterprise procedures. Results are generally interpreted and reported to supervising scientists, medical, veterinary or other responsible officers of an enterprise, regulatory authority or legal agency.Industry representatives have provided case studies to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. These can be found at the end of this unit of competency under the section 'This competency in practice'. | ||
Duration and Setting | X weeks, nominally xx hours, delivered in a classroom/online/blended learning setting. |
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Prerequisites/co-requisites | MSL974006A Perform biological proceduresMSL973007A Perform microscopic examinationMSL973004A Perform aseptic techniques | ||
Competency Field |
Development and validation strategy and guide for assessors and learners | Student Learning Resources | Handouts Activities |
Slides PPT |
Assessment 1 | Assessment 2 | Assessment 3 | Assessment 4 | |
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Elements of Competency | Performance Criteria | |||||||
Element: Interpret and schedule test requirements |
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Element: Receive and handle samples |
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Element: Prepare equipment and reagents |
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Element: Extract, verify and manipulate biomolecules |
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Element: Process data |
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Element: Maintain a safe work environment |
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Element: Report and communicate results |
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Evidence Required
List the assessment methods to be used and the context and resources required for assessment. Copy and paste the relevant sections from the evidence guide below and then re-write these in plain English.
The Evidence Guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package. | |
Overview of assessment | |
Critical aspects for assessment and evidence required to demonstrate competency in this unit | Assessors should ensure that candidates can: conduct work practices in an ethical and professional manner and in accordance with relevant legislation, regulation and codes of practice maintain security, integrity, traceability and identity of samples, sub-samples and work records obtain purified biomolecules from samples prevent/minimise DNA/RNA contamination perform tests/procedures, such as PCR, ligation and restriction enzyme digestion with appropriate controls follow enterprise safety standards, procedures and practices follow enterprise procedures and test methods consistently and accurately operate test equipment to enterprise standards and/or manufacturer's specification identify atypical results as out of normal range or an artefact trace and source obvious causes of artefacts communicate identified problems to a supervisor record and communicate results as per enterprise procedures. |
Context of and specific resources for assessment | This unit of competency is to be assessed in the workplace or simulated workplace environment. This unit of competency may be assessed with: MSL974008A Capture and manage scientific images MSL975001A Perform microbiological tests MSL975008A Apply electrophoretic techniques MSL975009A Apply routine chromatographic techniques MSL975013A Perform tissue and cell culture techniques MSL975020A Apply routine spectrometric techniques. Resources may include: laboratory equipped with appropriate test equipment/instruments, standards and reagents enterprise procedures, standard methods, manuals and supplier documentation. |
Method of assessment | The following assessment methods are suggested: review of test records and workplace documentation completed by the candidate review of results obtained by the candidate over a period of time to ensure accurate and consistent results are obtained within required timelines observation of candidate isolating, purifying, verifying and manipulating biomolecules oral or written questioning feedback from peers and supervisors. In all cases, practical assessment should be supported by questions to assess underpinning knowledge and those aspects of competency which are difficult to assess directly. Where applicable, reasonable adjustment must be made to work environments and training situations to accommodate ethnicity, age, gender, demographics and disability. Access must be provided to appropriate learning and/or assessment support when required. The language, literacy and numeracy demands of assessment should not be greater than those required to undertake the unit of competency in a work like environment. |
This competency in practice | Industry representatives have provided the case studies below to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. Biomedical As part of a diagnostic service to verify progenitor status of livestock, a technician is required to extract DNA from a blood sample, perform the PCR to amplify micro-satellite DNA and prepare the sample for DNA electrophoresis and fragment size analysis. The technician provides documentation to meet evidentiary standards. The technician understands the implications of the tests for the client and is careful to ensure the sample can be traced from the source, that no contamination takes place and that the results are kept confidential. Food processing A meat export company has commissioned a study of the effectiveness of the introduction of a 'cold-chain' process to a client country. The company requires rapid results. As part of the monitoring team, a technician is required to perform routine testing of surface swabs of meat samples for bacterial contamination using a PCR analytic technique. Although the tests are quite routine, the technician pays close attention to all aspects of the work as the consequences of invalid results would be severe for the company and laboratory. The technician also keeps comprehensive work records and maintains strict confidentiality. |
Submission Requirements
List each assessment task's title, type (eg project, observation/demonstration, essay, assignment, checklist) and due date here
Assessment task 1: [title] Due date:
(add new lines for each of the assessment tasks)
Assessment Tasks
Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.
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Required skills |
Required skills include: conducting work practices in an ethical and professional manner and in accordance with relevant legislation, regulation and codes of practice maintaining security, integrity, traceability and identity of samples, sub-samples and work records performing molecular biology tests and procedures following enterprise safety standards, procedures and practices identifying atypical results as out of normal range or an artefact tracing and sourcing obvious causes of artefacts communicating identified problems to a supervisor recording results according to enterprise procedures |
Required knowledge |
Required knowledge includes: hazards and risks in molecular biology laboratories common biotechnology terms molecular biology principles and concepts underpinning tests/procedures, such as: DNA and RNA structure and function protein structure and function relationship between chemical and physical properties of nucleic acids and proteins and the techniques used for sampling, preparation and testing replication transcription, translation and gene regulation relationship between structure, organisation and function of biomolecules to the storage of information in cells, chromatin, circular and linear chromosomes, RNA, genes and plasmids molecular genetics (molecular nature, organisation and function of genes) molecular mechanisms of DNA mutation and variation DNA transfer in prokaryotes (transformation, conjugation and transduction) restriction enzyme and ligase structure, nomenclature, function, specificity and stability, and cohesive versus blunt ends ethical issues associated with biotechnology, such as: use of animals for research genetic modification of organisms and food use of gene therapy, cloning and stem cells in vitro fertilisation forensic testing of populations importance of commercial confidentiality, protection of intellectual property and patents genetic screening of humans sex determination and parentage testing of embryos/humans importance and appropriate use of validation methods, controls and certified reference materials enterprise and/or legal traceability requirements relevant health, safety and environment requirements |
The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included. | |
Codes of practice | Where reference is made to industry codes of practice, and/or Australian/international standards, it is expected the latest version will be used |
Standards, codes, procedures and/or enterprise requirements | Standards, codes, procedures and/or enterprise requirements may include: Australian and international standards, such as: AS 2162.1-1996 Verification and use of volumetric apparatus - General - Volumetric glassware AS 2252 Biological safety cabinets AS 3753-2001 Recommended practice for chemical analysis by ultraviolet/visible spectrophotometry AS ISO 17025-2005 General requirements for the competence of testing and calibration laboratories AS/NZS 1269 Set:2005 Occupational noise management set AS/NZS 1337 Eye protection AS/NZS 2161 Set:2008 Occupational protective gloves set AS/NZS 2210:1994 Occupational protective footwear AS/NZS 2243.3:2002 Safety in laboratories - Microbiological aspects and containment facilities AS/NZS 4501 Set:2008 Occupational clothing set HB 9-1994 Occupational personal protection Australian code of good manufacturing practice for medicinal products (GMP) calibration and maintenance schedules cleaning, hygiene, personal hygiene requirements enterprise procedures, standard operating procedures (SOPs) and operating manuals equipment startup, operation and shutdown procedures European Union (EU) Guide to physical containment levels and facility types guidelines for small scale genetic manipulation work from the gene technology regulations incident and accident/injury reports instructions to comply with new legislation, standards, guidelines and codes material, production and product specifications National Association of Testing Authorities (NATA) Accreditation programs requirements National Health and Medical Research Council (NHMRC) National Registration Authority (NRA) principles of good laboratory practice (GLP) production and laboratory schedules quality manuals and equipment and procedure manuals quality system and continued improvement processes safety requirements for equipment, materials or products and material safety data sheets (MSDS) sampling procedures (labelling, preparation, storage, transport and disposal) schematics, work flows and laboratory layouts test procedures (validated and authorised) Therapeutic Goods Regulations 1009 United States Food and Drug Administration (USFDA) validated and authorised test methods waste minimisation, containment, processing and disposal procedures World Health Organisation (WHO) |
Hazards | Hazards may include: electric shock (e.g. electrophoresis power packs) microbiological organisms and agents associated with soil, air, water, blood and blood products, and human or animal tissue and fluids chemicals, such as acrylamide, temed, phenol and ammonium persulphate mutagens, such as ethidium bromide, tumour promoters and cytotoxic materials genetically altered organisms, transformed cultures and organisms allergenic proteins radioisotopes transilluminators and other ultraviolet (UV) light sources aerosols from broken centrifuge tubes and pipetting sharps and broken glassware flammable liquids and gases cryogenics, such as dry ice and liquid nitrogen disturbance or interruption of services |
Safe work practices and hazard control measures | Safe work practices and hazard control measures may include: ensuring access to service shut-off points recognising and observing hazard warnings and safety signs labelling of samples, reagents, aliquoted samples and hazardous materials handling and storage of hazardous materials and equipment in accordance with labelling, MSDS and manufacturer's instructions identifying and reporting operating problems or equipment malfunctions cleaning and decontaminating equipment and work areas regularly using enterprise procedures using personal protective clothing and equipment, such as gloves, safety glasses, coveralls, gowns, body suits and respirators using containment facilities (PCII, PCIII and PCIV physical containment laboratories), containment equipment (biohazard containers, laminar flow cabinets, Class I, II and III biohazard cabinets) and containment procedures reporting abnormal emissions, discharges and airborne contaminants, such as noise, light, solids, liquids, water/waste water, gases, smoke, vapour, fumes, odour and particulates to appropriate personnel |
Equipment and instrumentation | Equipment and instrumentation may include: pipettes, tubes and racks heating blocks and polymerase chain reaction (PCR) thermal cyclers swabs centrifuges and shakers electrophoresis tanks and power supplies incubation cabinets for micro-organisms and cell culture liquid nitrogen containers autoclaves water baths waste containers fumehoods analytical instruments, such as spectrophotometers |
Reagents | Reagents may include: DNA, RNA and proteins enzymes (restriction, ligation and polymerisation) buffers agarose, starch and polyacrylamide for electrophoresis gels commercial kits for extraction and manipulation of DNA/RNA phenol and chloroform ethidium bromide cell and culture media DNA, and protein stains specialised probe materials, such as radioactive, chemical and chemiluminescent labels blotting membranes chromatographic media |
Molecular biology tests and procedures | Molecular biology tests and procedures may include: generic skills: sample digestion, extraction, filtration, separation, dialysis, precipitation and centrifugation accurate and reliable use of micropipettes application of aseptic techniques labelling (e.g. digoxin, fluorescence, enzymes, radioactivity and antibodies) production, labelling and use of DNA probes preparation of competent bacterial cells preservation and storage of samples (e.g. freezing) extraction of nucleic acids: isolation of genomic and plasmid DNA and RNA from samples, such as plants, bacterial suspensions, white blood cells, cheek cells, animal and plant tissue, cultured cells and forensic specimens mini-prep and rapid method isolation of plasmid DNA purification of nucleic acids and proteins: purification of DNA using cesium gradients, commercial purification buffer kits and columns purification of recombinant protein by chromatography production of nucleic acids: amplification of DNA by polymerase chain reaction transformation with recombinant DNA identification of transformed organisms with appropriate selection and analytical techniques, such as selective media and insertional inactivation use of enzymes: storage and handling of enzymes taking into account segregation, temperature, buffers and labelling to avoid wastage, denaturation and contamination ligation assistance with analysis of nucleic acids and proteins: sequencing DNA assaying of DNA purity and concentration using spectrometric analysis electrophoresis of restriction enzyme digests of plasmid and genomic DNA using agarose gel DNA sequencing by Sanger method testing using restriction fragment length polymorphism (RFLP), probes and microsatellites detection of protein products by measuring activity, including a range of immunological assays assistance with hybridisations: hybridisation to screen cDNA libraries blotting (southern blots for DNA and Western blots for protein) cloning: cloning and sub-cloning of genes and fragments of DNA applications of techniques: PCR methods to detect gene expression, such as RNA hybridisation, immunological techniques and radioactive labelling testing DNA for sequence variation that is either causative of, or associated with, human disease testing blood for the presence of viruses using the polymerase chain reaction identification of species, such as bacterial contaminants generating data for taxonomic and ecological investigations |
Corrective action | Corrective action may involve: purification dilution additional extraction steps |
Records | Records may include: test and calibration results equipment use, maintenance and servicing history photo images of gels, radioisotopes and digital images chain of custody from sample to result supplier certificates of analysis quality control/analysis data |
Occupational health and safety (OHS) and environmental management requirements | OHS and environmental management requirements: all operations must comply with enterprise OHS and environmental management requirements, which may be imposed through state/territory or federal legislation - these requirements must not be compromised at any time all operations assume the potentially hazardous nature of samples and require standard precautions to be applied where relevant, users should access and apply current industry understanding of infection control issued by the National Health and Medical Research Council (NHMRC) and State and Territory Departments of Health |
Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.
Observation Checklist
Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice | Yes | No | Comments/feedback |
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Review test request to identify samples to be tested, test method and equipment/instruments involved | |||
Identify hazards and enterprise control measures associated with the sample, preparation methods, reagents and/or equipment | |||
Log and label samples according to enterprise procedures | |||
Record sample description, compare with specification and note and report discrepancies | |||
Store samples in accordance with enterprise and test method requirements | |||
Maintain chain of custody, traceable to the worker, for all samples | |||
Set up equipment/instrumentation in accordance with test method requirements and perform pre-use and safety checks | |||
Select and collect reagents in accordance with test method requirements | |||
Prepare and label reagents in accordance with test method requirements | |||
Produce/extract biomolecules from samples using appropriate isolation methods | |||
Prevent contamination of samples by unwanted biomolecules | |||
Recognise the presence of common inhibitors of biomolecular reactions and take corrective action | |||
Quantify and qualify biomolecular yields from purified extractions | |||
Use appropriate techniques to prepare and test a range of biomolecular samples | |||
Use controls and reference standards to confirm the integrity of biomolecular sample preparation and procedures | |||
Record test data noting atypical observations | |||
Ensure results are consistent with reference standards and expectations | |||
Record and report results in accordance with test methods | |||
Interpret trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel | |||
Troubleshoot basic procedure, reagent or equipment problems which have led to atypical data or results | |||
Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel | |||
Minimise the generation of wastes | |||
Ensure the safe disposal of wastes, including hazardous wastes and tested samples | |||
Clean, care for and store equipment and reagents | |||
Record approved data into enterprise system | |||
Keep accurate, traceable work records to protect the enterprise's intellectual property rights | |||
Maintain confidentiality and security of enterprise information and laboratory data | |||
Maintain equipment logs in accordance with enterprise procedures |
Forms
Assessment Cover Sheet
MSL975014A - Perform molecular biology tests and procedures
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Assessment Record Sheet
MSL975014A - Perform molecular biology tests and procedures
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